General Cell Lines and Cell Cultures
Eurofins Discoverx ChemiBrite FFA1/GPR40 Free Fatty Acid Receptor Stable Cell Line, 2 vials. (HTS038L)
The ChemiBrite FFA1/GPR40 Free Fatty Acid Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. free fatty acids) based activation of FFAR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses FFAR1, a naturally Gq coupled GPCR. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of FFAR1, the resulting calcium activity can be assayed in either detection mode. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay NOP/ORL1 Opioid Receptor Frozen Cells, 2 Vials of Cells. (HTS040RTA)
The Ready-to-Assay NOP/ORL1 Opioid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. orphanin FQ) based activation of OPRL1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses OPRL1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of OPRL1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen AT1 Angiotesin Receptor Stable Cell Line, 2 vials. (HTS064C)
The ChemiScreen AT1 Angiotensin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. Angiotensin-II) based activation of AGTR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses AGTR1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of AGTR1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen CCR9 Chemokine Receptor Membrane Preparation, 1 vial. (HTS036M)
The ChemiScreen CCR9 Chemokine Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CCR9 surface expression. ChemiScreen membrane preps can be used to screen for GPCR activity using GTPγS assays. Increased GPCR activation levels will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen NMU1 Neuromedin U Receptor Stable Cell Line, 2 vials. (HTS062C)
The ChemiScreen nMU1 Neuromedin U Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. Neuromedin U) based activation of nMU1R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses nMU1R alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of nMU1R, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay CysLT1 Leukotriene Receptor Frozen Cells, 2 Vials of Cells. (HTS061LRTA)
The Ready-to-Assay CysLT1 Leukotriene Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. leukotriene) based activation of CysLT1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CysLT1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of CysLT1, the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen MT1 Melatonin Receptor Membrane Preparation, 1 vial. (HTS065M)
The ChemiScreen MT1 Melatonin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of MTNR1A surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen SST2 Somatostatin Receptor Stable Cell Line, 2 vials. (HTS028C)
The ChemiScreen SST2 Somatostatin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. somatostatin) based activation of SSTR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses SSTR2 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of SSTR2, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen CysLT1 Leukotriene Receptor Stable Cell Line, 2 vials. (HTS061C)
The ChemiScreen CysLT1 Leukotriene Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. leukotriene) based activation of CysLT1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CysLT1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CysLT1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen CCR9 Chemokine Receptor Stable Cell Line, 2 vials. (HTS036C)
The ChemiScreen CCR9 Chemokine Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CCL25/TECK) based activation of CCR9 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CCR9 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CCR9, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen MC5 Melanocortin Receptor Stable Cell Line, 2 vials. (HTS155C)
The ChemiScreen MC5 Melanocortin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. α-MSH) based activation of MC5R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses MC5R alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of MC5R, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen mGLU2 Metabotropic Glutamate Receptor Membrane Preparation, 1 vial. (HTS146M)
The ChemiScreen mGLU2 Metabotropic Glutamate Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of GRM2 surface expression. ChemiScreen membrane preps can be used to screen for GPCR activity using GTPγS assays. Increased GPCR activation levels will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx Ready-to-Assay GLP-1 Receptor Frozen Cells, 2 Vials of Cells. (HTS163LRTA)
The Ready-to-Assay GLP-1 Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. GLP-1) based activation of GLP1R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GLP1R alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of GLP1R, the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay TSH Glycoprotein Hormone Receptor Frozen Cells, 2 Vials of Cells. (HTS133RTA)
The Ready-to-Assay TSH Glycoprotein Hormone Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. TSH) based activation of TSHR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses TSHR alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of TSHR, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.